更新時(shí)間：2023-11-02 
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Transwell Invasion Assay
A Transwell chamber with an 8-μm pore polycarbonate membrane (Biofil, Guangzhou, China) was used for the invasion assay. A medium containing 10% fetal bovine serum was added to the lower chamber. The cells were suspended in a serum-free medium and inoculated into the upper chamber coated with a matrix gel (ABW, Shanghai, China). After incubation for 24 h, the cells in the upper chamber were scraped with a cotton swab, fixed on the lower surface of the membrane with paraformaldehyde for 30 min, and stained with crystal violet (Solarbio) for 30 min. The cells were counted after capturing the images.
Tube Formation Assay
The 96-well plates coated with matrix gel (ABW) were incubated at 37 °C in 5% CO2. After 30 min, 50 μL of cell suspension was added to each well. Images were captured after 4 h.
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